CD55 Antibody / DAF
Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF) . CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The mAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH) .
Product Specifications
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P08174
Host
Mouse
Reactivity
Human
Immunogen
PHA stimulated human PBL were used as the immunogen for the CD55 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
143-30
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD55 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD55 antibody is available for research use only.
Storage Conditions
Store the CD55 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd55-antibody-daf-143-30-v2475
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD55 antibody should be determined by the researcher.
CAS Number
9007-83-4
Location
Cell surface
Image Legend
Flow cytometry testing of PFA-fixed human HeLa cells with CD55 antibody (clone 143-30) ; Red=isotype control, Blue= CD55 antibody.
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
