Calponin Antibody
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34 kDa form and a 29kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P51911
Host
Mouse
Reactivity
Human, Rat
Immunogen
Crude human uterus extract was used as the immunogen for the Calponin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CALP
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Calponin antibody is available for research use only.
Storage Conditions
Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Western blot testing of human endometrium tissue lysate with Calponin antibody. Predicted molecular weight ~34 kDa.
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