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CD45RO Antibody

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

P08575

Host

Mouse

Reactivity

Human

Immunogen

Human T lymphocytes were used as immunogen for this CD45RO antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

T200/797

Applications

FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

The CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This CD45RO antibody is available for research use only.

Storage Conditions

The CD45RO antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface

Image Legend

IHC testing of CD45RO antibody and FFPE human tonsil. HIER: boil tissue sections in 10mM citrate buffer, pH 6 for 10-20 min followed by cooling at RT for 20 min.

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