CD45 Antibody
Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. CD45RA contains the A exon and is a naive T-cell marker which may help prevent autoimmune disease. CD45RB contains B and stains most leukemias and lymphomas. CD45RC contains C and stains thymocytes, monocytes and dendritic cells. CD45RO doesn't contain A, B or C and is a marker of activated T-cells that can be used to classify and diagnose and classify lymphomas. This antibody will bind to all CD45 isoforms. The variation in these isoforms is localized to the extracellular domain, with the intracellular domain being conserved. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Product Specifications
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P08575
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the CD45 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
135-4C5
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
CD45 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD45 antibody is available for research use only.
Storage Conditions
CD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd45-antibody-135-4c5-v2399
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
CAS Number
9007-83-4
Location
Cell surface and cytoplasmic
Image Legend
Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45 antibody (clone 135-4C5, green) and Reddot nuclear stain (red) .
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