CD19 Antibody
CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]
Product Specifications
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P15391
Host
Mouse
Reactivity
Human
Immunogen
Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CVID3/429
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD19 antibody is available for research use only.
Storage Conditions
CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd19-antibody-cvid3429-v2389
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.
CAS Number
9007-83-4
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red) .
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