CD19 Antibody
CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P15391
Host
Mouse
Reactivity
Human
Immunogen
Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CVID3/429
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD19 antibody is available for research use only.
Storage Conditions
CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red) .
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