Anti-CD19 Antibody

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. It is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

P15391

Host

Mouse

Reactivity

Human

Immunogen

Human CD19 recombinant protein was used as immunogen for this anti-CD19 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CVID3/155

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Anti-CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This anti-CD19 antibody is available for research use only.

Storage Conditions

Anti-CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD19 antibody to be titered up or down for optimal performance.

Location

Cell surface, cytoplasmic

Image Legend

Flow cytometry testing of lymphocyte-gated human PBM cells with anti-CD19 antibody (clone CVID3/155) ; Gray=unstained cells, Orange= anti-CD19 antibody stained cells.