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CD8a Antibody

CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked heterodimer or homodimer. CD8 is expressed on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

UniProt

P01732

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as immunogen for this CD8a antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

RIV11

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

CD8a antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This CD8a antibody is available for research use only.

Storage Conditions

CD8a antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.

Location

Cell surface

Image Legend

Flow cytometry staining of human PBM cells with CD8a antibody (clone RIV11) ; Red=isotype control, Blue= SLC16A11 antibody.

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