Anti-CD8 Antibody (alpha)

Cluster of Differentiation 8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD4 and 8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/million cells

UniProt

P01732

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CD8a protein was used as immunogen for this anti-CD8 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C8/1035

Applications

ELISA, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the anti-CD8 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This anti-CD8 antibody is available for research use only.

Storage Conditions

Store the anti-CD8 antibody (with azide) at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD8 antibody to be titered up or down for optimal performance.