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CD4 Antibody

CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/million cells

UniProt

P01730

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CD4 protein was used as immunogen for the CD4 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C4/206

Applications

ELISA, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

CD4 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

The CD4 antibody is available for research use only.

Storage Conditions

CD4 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the CD4 antibody for a specific application should be determined by researcher.

Location

Cell surface

Image Legend

Flow cytometry staining of lymphocyte gated human PBM cells with CD4 antibody; Gray=unstained, Green= CD4 antibody.

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