CD3 Antibody / CD3e
The CD3 complex mediates signal transduction. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P07766
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Human peripheral lymphocytes were used as immunogen for the CD3 antibody. The epitope is known to be on the epsilon chain.
Clonality
Monoclonal
Isotype
IgG3 κ
Clone
RIV9
Applications
Functional studies, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
CD3 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
The CD3 antibody is available for research use only.
Storage Conditions
CD3 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.
Location
Cell surface and cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone RIV9) and Reddot nuclear stain (red) .
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