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CD3 Antibody / CD3e

The CD3 complex mediates signal transduction. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

P07766

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Human peripheral lymphocytes were used as immunogen for the CD3 antibody. The epitope is known to be on the epsilon chain.

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

RIV9

Applications

Functional studies, FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

CD3 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

The CD3 antibody is available for research use only.

Storage Conditions

CD3 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.

Location

Cell surface and cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone RIV9) and Reddot nuclear stain (red) .

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