CD3 Antibody
CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
Human T cell leukemia cells were used as immunogen for the CD3 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
B-B12
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
CD3 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
The CD3 antibody is available for research use only.
Storage Conditions
CD3 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.
Location
Cell surface and cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone B-B12) and Reddot nuclear stain (red) .
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