HER-2 Antibody
ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ERB2/776
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HER-2 antibody at 2-8°C.
Limitations
This HER-2 antibody is available for research use only.
Storage Conditions
Store the HER-2 antibody at 2-8°C.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Extracellular/Intracellular cell membrane (this mAb is binds to the intracellular portion of the protein)
Image Legend
Immunofluorescent staining of MeOH-fixed human MCF7 cells with HER-2 antibody (clone ERB2/776, green) and Reddot nuclear stain (red) .
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