TAG-72 Antibody
This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this TAG-72 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
B72.3
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the TAG-72 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TAG-72 antibody is available for research use only.
Storage Conditions
Store the TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic and cell surface
Image Legend
IHC staining of human ovarian carcinoma with TAG-72 antibody (clone B72.3) .
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