Nucleoli Marker Antibody / Human Nucleolar Antigen

This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells , Immunofluorescence: 1-2 µg/mL, Immunocytochemistry: 1-2 µg/mL for 30 min at RT, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of myeloid leukemia biopsy cells were used as the immunogen.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NM95

Applications

FACS, IF, ICC, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Nucleoli Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Nucleoli Marker antibody is available for research use only.

Storage Conditions

Store the Nucleoli Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Nucleoli

Image Legend

IHC staining of FFPE human skin with Nucleoli Marker antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.