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Myeloid Cell Marker Antibody

This antibody recognizes a 183kDa protein with DNA-binding characteristics, which is identified as a myeloid cell specific antigen. Clone BM-1 antibody reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. This type of marker is useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. This mAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of the marker protein is not clear, although it has been proposed that it may play a role in cell differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

Human peripheral blood mononuclear cells were used as the immunogen for this Myeloid cell marker antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BM-1

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Myeloid cell marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Myeloid cell marker antibody is available for research use only.

Storage Conditions

Store the Myeloid cell marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic and nuclear

Image Legend

Formalin/paraffin human tonsil stained with Myeloid cell marker antibody (BM-1) .

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