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Mitochondrial Antibody

MAb MTC719 recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Host

Mouse

Reactivity

Human

Immunogen

The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MTC719

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Mitochondrial antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Mitochondrial antibody is available for research use only.

Storage Conditions

Store the Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Mitochondria in cytoplasm

Image Legend

IHC testing of FFPE human bladder carcinoma with Mitochondrial antibody (clone MTC719) .

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