Mitochondrial Antibody
MAb MTC719 recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MTC719
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Mitochondrial antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Mitochondrial antibody is available for research use only.
Storage Conditions
Store the Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Mitochondria in cytoplasm
Image Legend
IHC testing of FFPE human bladder carcinoma with Mitochondrial antibody (clone MTC719) .
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