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Mitochondria Antibody

Clone MTC02 antibody recognizes a unique 60kDa antigen that is present only on mitochondria in human cells. It is a part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This antibody recognizes an antigen associated with the mitochondria specific to human cells alone. MTC02 mAb can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MTC02 antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MTC02

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Mitochondria antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Mitochondria antibody is available for research use only.

Storage Conditions

Store the Mitochondria antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Mitochondria in cytoplasm

Image Legend

IHC testing of FFPE human tonsil with Mitochondria antibody

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