Mitochondria Antibody
Clone MTC02 antibody recognizes a unique 60kDa antigen that is present only on mitochondria in human cells. It is a part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This antibody recognizes an antigen associated with the mitochondria specific to human cells alone. MTC02 mAb can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MTC02 antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MTC02
Applications
IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Mitochondria antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Mitochondria antibody is available for research use only.
Storage Conditions
Store the Mitochondria antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Mitochondria in cytoplasm
Image Legend
IHC testing of FFPE human tonsil with Mitochondria antibody
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