IL-6 Antibody

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Neutralization Studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant mouse IL-6 protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

MP5-20F3

Applications

Neutralization Studies, FACS, IF

Purity

Protein G purified IL-6 antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the IL-6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This IL-6 antibody is available for research use only.

Storage Conditions

Store the IL-6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IL-6 antibody to be titered up or down for optimal performance.