Human Nuclear Antigen Antibody PE Conjugate

This human nuclear marker/antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The human nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood, Immunofluorescence : 1:50-1:100 for 30 minutes at RT

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

235-1

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

PE Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the human Nuclear Antigen antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

Limitations

This Nuclear Antigen antibody is available for research use only.

Storage Conditions

Store the human Nuclear Antigen antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear Antigen antibody to be titered up or down for optimal performance.