Human Nuclear Antigen Antibody
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1-2 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
235-1
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Human Nuclear Antigen antibody at 2-8oC, protected from light.
Limitations
This Human Nuclear Antigen antibody is available for research use only.
Storage Conditions
Store the Human Nuclear Antigen antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Human Nuclear Antigen antibody should be determined by the researcher.
Location
Nuclear
Image Legend
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