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Human Nuclear Antigen Antibody

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

235-1

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

CF488 Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Human Nuclear Antigen antibody at 2-8oC, protected from light.

Limitations

This Human Nuclear Antigen antibody is available for research use only.

Storage Conditions

Store the Human Nuclear Antigen antibody at 2-8°C, protected from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Human Nuclear Antigen antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Intracellular flow cytometry testing of human 293T cells with Human Nuclear Antigen antibody; Black=cells alone, Gray=isotype control, Green= Human Nuclear Antigen antibody.
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