Hep Par 1 Antibody / Hepatocyte Paraffin 1

Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Host

Mouse

Reactivity

Human

Immunogen

Extract of a formalin-fixed, rejected-allograft of a human liver was used as the immunogen for this Hep Par 1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

OCH1E5

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Hep Par 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Hep Par 1 antibody is available for research use only.

Storage Conditions

Store the Hep Par 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Finely granular cytoplasmic

Image Legend

IHC staining of human hepatocellular carcinoma with Hep Par 1 antibody (clone OCH1E5) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.