Erythrocyte Specific Antigen Antibody

This antibody detects an erythrocyte specific antigen. The antibody shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the erythrocyte specific antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This antibody is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Hepatocytes from a 20-22 week-old human fetus were used as the antigen for this Erythrocyte Specific Antigen antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SFL23.6

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Erythrocyte Specific Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Erythrocyte Specific Antigen antibody is available for research use only.

Storage Conditions

Store the Erythrocyte Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Erythrocyte Specific Antigen antibody to be titered up or down for optimal performance.