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Basic Cytokeratin Antibody

6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (Cat No V2330) .Cat No V2330

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AE3

Applications

WB, FACS, IHC-P, IF

Purity

Protein G purified

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Basic Cytokeratin antibody AE3 is available for research use only. The formulation is not suitable for therapeutic or diagnostic testing.

Storage Conditions

Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC: FFPE skin stained with Basic Cytokeratin antibody AE3.

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