HMW Cytokeratin Antibody / Basic / Type II
This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
Product Specifications
CAS Number
9007-83-4
Specifications
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
34BE12
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HMW Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HMW Cytokeratin antibody is available for research use only.
Storage Conditions
Store the HMW Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human MCF7 cell lysate with HMW Cytokeratin antibody (clone 34BE12) .
Documents
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
S-phase Kinase-associated Protein 2 (SKP2, Cyclin-A/CDK2-associated Protein p45, F-box Protein Skp2, F-box/LRR-repeat Protein 1, FBL1, FBXL1, FLB1, MGC1366, p45skp2)