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BrdU Antibody

This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Mouse

Reactivity

All Species Expected

Immunogen

BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

BRD494

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BrdU / Bromodeoxyuridine antibody is available for research use only.

Storage Conditions

Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Nuclear

Image Legend

IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD494) .

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