Bromodeoxyuridine Antibody
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BRD469
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Bromodeoxyuridine antibody at 2-8oC, protected from light.
Limitations
This Bromodeoxyuridine antibody is available for research use only.
Storage Conditions
Store the Bromodeoxyuridine antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Bromodeoxyuridine antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC/IF staining of FFPE rat kidney tissue with CF488-conjugated BrdU antibody.
Documents
Frequently Asked Questions
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