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Bromodeoxyuridine Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine conjugated to KLH was used as the immunogen.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BRD469

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

CF488 Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Bromodeoxyuridine antibody at 2-8oC, protected from light.

Limitations

This Bromodeoxyuridine antibody is available for research use only.

Storage Conditions

Store the Bromodeoxyuridine antibody at 2-8°C, protected from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Bromodeoxyuridine antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC/IF staining of FFPE rat kidney tissue with CF488-conjugated BrdU antibody.

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