What is the catalog number for BrdU Antibody?

The catalog number for BrdU Antibody is V2321-20UG.

What sizes are available for BrdU Antibody?

BrdU Antibody is available in the following size(s): 20 µg.

Is BrdU Antibody in stock?

BrdU Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-3 µg/mL

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine conjugated to KLH was used as the immunogen.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BRD469

Applications

IHC-P, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BrdU antibody is available for research use only.

Storage Conditions

Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.</br></p>