Muscle Actin Antibody (pan)
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P62736, P68032, P68133
Host
Mouse
Reactivity
Human, Mouse
Immunogen
SDS extract of human myocardium was used as the immunogen for this Muscle Actin HHF35 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HHF35
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Muscle Actin antibody is available for research use only.
Storage Conditions
Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC staining of human leiomyosarcoma (10X) with Muscle Actin antibody (HHF35) .
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