TMEM16A Antibody (DOG1)
Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for TMEM16A has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 904-986 from the human TMEM16A/DOG1 protein was used as the immunogen for this TMEM16A antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
DG1/447
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the TMEM16A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TMEM16A antibody is available for research use only.
Storage Conditions
Store the TMEM16A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cell Surface and Cytoplasmic
Image Legend
Formalin/paraffin human gastrointestinal stromal tumor (10X) stained with TMEM16A antibody (clone DG1/447) .
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