DOG1 Antibody / TMEM16A / ANO1
Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for TMEM16A has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 904-986 from the human TMEM16A/DOG1 protein was used as the immunogen for this TMEM16A antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
DG1/447
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the DOG1 antibody at 2-8oC, protected from light.
Limitations
This DOG1 antibody is available for research use only.
Storage Conditions
Store the DOG1 antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the DOG1 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
SDS-PAGE analysis of purified, BSA-free DOG1 antibody (clone DG1/447) as confirmation of integrity and purity.
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