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UchL1 Antibody

This antibody specifically reacts with a protein of 20-30kDa, identified as UchL1 (Ubiquitin carboxyl-terminal hydrolase-1), or PGP9.5. Studies using UchL1 antibody have shown that expression is related to tumor progression, with high expression correlating with poor prognosis, making the protein a potentially useful marker for pancreatic and invasive colorectal cancer. A mutation in gene is believed to cause a form of Parkinson's disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Native protein from brain was used as the immunogen for this UchL1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

13C4

Applications

WB, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the UchL1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This UchL1 antibody is available for research use only.

Storage Conditions

Store the UchL1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the UchL1 antibody to be titered up or down for optimal performance.

Location

Cytoplasmic

Image Legend

Western blot testing of human brain lysate with UchL1 antibody (clone 13C4) . Predicted molecular weight ~25 kDa.

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