TTF-1 Antibody / NKX2.1
This mAb recognizes a protein of 40kDa, identified as Thyroid transcription factor 1. TTF1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. antibody to TTF1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. The antibody can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF1 reactivity is also seen in thyroid malignancies.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Rat full length recombinant protein was used as the immunogen for this TTF-1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
8G7G3/1
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TTF-1 antibody is available for research use only.
Storage Conditions
Store the TTF-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC staining of human lung adenocarcinoma (10X) with TTF-1 antibody (8G7G3/1) .
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