TGF alpha Antibody

TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgM k

Clone

1E8-G6

Applications

FACS, IF, IHC-P

Purity

PEG precipitation

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TGF alpha antibody is available for research use only.

Storage Conditions

Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.