TGF alpha Antibody
TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MF9
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TGF alpha antibody is available for research use only.
Storage Conditions
Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/tgf-alpha-antibody-mf9-v2266
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Location
Cytoplasmic and secreted
Image Legend
IHC testing of FFPE human testicular carcinoma with TGF alpha antibody
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