Transferrin Receptor Antibody / CD71

This antibody recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV) . The antibody is highly specific to transferrin receptor and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL, Western blot: 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

The KG1 acute myeloid leukemia cell line was used as the immunogen for this Transferrin receptor antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

DF1513

Applications

FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Transferrin receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Transferrin receptor antibody is available for research use only.

Storage Conditions

Store the Transferrin receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Location

Cell surface

Image Legend

Western blot testing of human 1) Jurkat, 2) Raji and 3) ThP-1 cell lysate with Transferrin receptor antibody (clone DF1513) . Predicted molecular weight: 85-95 kDa.