Melanoma Antibody / gp100 / PMEL17
By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100) .
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
NKI-beteb
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Melanoma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Melanoma gp100 antibody is available for research use only.
Storage Conditions
Store the Melanoma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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