Melanoma Antibody / gp100 / PMEL17

By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100) .

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

NKI-beteb

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Melanoma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Melanoma gp100 antibody is available for research use only.

Storage Conditions

Store the Melanoma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

Location

Cytoplasmic

Image Legend

IHC testing of melanoma tissue stained with Melanoma antibody (NKI-beteb) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.