Progesterone Receptor Antibody

This antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa) . Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL for 2 hr at RT, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Progesterone Receptor protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PR501

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Progesterone receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Progesterone receptor antibody is available for research use only.

Storage Conditions

Store the Progesterone receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.