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PCNA Antibody

This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA) . PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PC10

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PCNA antibody is available for research use only.

Storage Conditions

Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Predominantly nuclear, some cytoplasmic

Image Legend

Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10) . Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.

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