PAX7 Antibody

The PAX gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the PAX family of proteins have been linked to disease and cancer in humans. PAX7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that PAX7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding PAX7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 0.5-1 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Western blot: 0.5-1 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PAX7/497

Applications

IF, FACS, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the PAX7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PAX7 antibody is available for research use only.

Storage Conditions

Store the PAX7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.