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Neurofilament Antibody / Heavy

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H) . The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Human recombinant Neurofilament protein was used as the immunogen. (1)

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NF421

Applications

WB, FACS, IF, IHC-P

Purity

Protein G purified Neurofilament antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Neurofilament antibody is available for research use only.

Storage Conditions

Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human cerebellum with Neurofilament antibody (clone NF421) .

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