MUC1 Antibody / Mucin-1
This antibody recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen) . The MUC1 antibody reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Human milk fat globule membranes were used as the immunogen for this MUC1 / Mucin-1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GP1.4
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MUC1 antibody is available for research use only.
Storage Conditions
Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic and cell surface
Image Legend
Western blot testing of human MCF7 cell lysate with MUC1 antibody (clone GP1.4) . This glycoprotein is commonly visualized between 120~500 kDa.
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