MUC18 Antibody / CD146

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. MUC18 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. MUC18 / CD146 is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this MUC18 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

OJ79c

Applications

FACS, IF

Purity

Protein G purified antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the MUC18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MUC18 antibody is available for research use only.

Storage Conditions

Store the MUC18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC18 antibody to be titered up or down for optimal performance.