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EpCAM Antibody PE Conjugate

Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9007-83-4

Specifications

FACS: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood), Immunofluorescence: 1:50-1:100

Host

Mouse

Reactivity

Human

Immunogen

Small cell lung carcinoma cells were used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VU-1D9

Applications

FACS

Purity

Protein G affinity chromatography

Format

PE Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the EpCAM antibody at 2-8°C. Conjugate is light-sensitive, protect from light.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at 2-8°C. Conjugate is light-sensitive, protect from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Location

Cell surface

Image Legend

Surface FACS staining of HT29 cells using PE conjugated EpCAM antibody (red) and isotype control antibody (green) .

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