EpCAM Antibody

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood, Immunofluorescence: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL at RT

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Small cell lung carcinoma cells were used as the immunogen for the EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VU-1D9

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the EpCAM antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human colon carcinoma with biotinylated EpCAM antibody (clone VU-1D9) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.