EpCAM Antibody
Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. The VU-1D9 antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
Small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody VU-1D9.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VU-1D9
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
EpCAM antibody
Location
Cell surface, cytoplasmic
Image Legend
IHC testing of FFPE colon carcinoma stained with EpCAM antibody (clone VU-1D9) .
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